Synthesis and Characterization of Silver Nanoparticles by Escherichia Coli

Abstract

This work investigates the environmentally friendly biosynthesis of AgNPs utilizing Escherichia coli. The bacterial strain was grown and then subjected to silver nitrate (AgNO?) for nanoparticle production in both the supernatant and pellet. As a result of surface plasmon resonance, the hue changed from light yellow to brown, confirming that AgNPs had been formed. The nanoparticles underwent X-ray diffraction and scanning electron microscopy characterization after being centrifuged to separate them. SEM showed spherical particles 5–25 nm in size, while XRD verified a crystalline structure with an average size of 118 nm. The work highlights the potential nanotechnology uses of proteins by emphasizing their function in stabilizing AgNPs.